Method for the estimation of N-acylated primary aromatic amines

Details Method for the estimation of N-acylated primary aromatic amines Method for the estimation of N-acylated primary aromatic amines

1981-12-01

1983-11-08

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435805, 435810, 435876, 435877, C12Q 134, C12R 139, C12R 140

Patent

active

044143273

ABSTRACT:
A method for the estimation of an anilide in which the anilide is first hydrolyzed enzymatically to an aniline and then the quantity of the aniline produced is estimated spectrophotometrically preferably colorimetrically.
The hydrolysis of the anilide may be catalyzed by any enzyme of the type, EC 3.5.1.13, known as aryl acylamidases. Preferably enzymes isolated from the cells of Pseudomonas fluorescens ATCC 39005 or Pseudomonas putida ATCC 39004 are employed.
The aniline may be analyzed, for example, by conversion to an indamine, an indophenol or an indoaniline, followed by colorimetric analysis of the colored quinone-type compound produced. This conversion may take place in the presence of an oxidizing agent, such as a copper (II) salt, and/or a base, such as ammonia.
A diagnostic kit to allow the routine use of the above method is also provided. In one embodiment of the kit, it comprises,

REFERENCES:
patent: 3986931 (1976-10-01), Bernt et al.
patent: 4336331 (1982-06-01), Nagasawa et al.
Hammond et al., The Lancet, 1(8216), 391-392 (1981).

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