Method for the enzymatic synthesis of oligonucleotides using the

Details Method for the enzymatic synthesis of oligonucleotides using the Method for the enzymatic synthesis of oligonucleotides using the

1994-06-13

1997-05-13

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 911, 435 6, 536 253, 536 256, C12P 1934, C12Q 168, C07H 2104, C07H 2100

Patent

active

056291779

ABSTRACT:
An oligonucleotide is synthesized by adding a 3'-phosphate blocked nucleotide to a primer, removing the blocking group from the primer-blocked nucleotide product using a thermostable 3'-phosphatase enzyme, and repeating these steps until the desired nucleotides have been added to the primer. A suitable phosphatase enzyme for use in this method is a thermostable phosphatase derived from the hyperthermophilic archaebacterium Pyrococcus furiosus.

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