Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1985-03-04
1987-03-24
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 35, 435 39, 435288, 435295, 435296, 435803, 436501, 436810, C12Q 104, C12Q 100, C12Q 116, C12Q 106, C12M 140, C12M 130, C12M 124, G01N 33566
Patent
active
046525211
ABSTRACT:
A method for the early diagnosis of Bordetella diseases based on the detection of Bordetella adenylate cyclase and the ability of this enzyme to catalyze the formation of cyclic adenosine monophosphate (cAMP). A sample secretion is taken from a suspected victim of a Bordetella disease (the most common of which in humans is whooping cough) and incubated under conditions to promote the formation of cAMP, which may then be measured as evidence of the presence of the disease. A sample of nasopharyngeal secretions is maintained in a nutrient medium pending assay. An adenylate cyclase assay is made by incubating the sample with adenosine triphosphate (ATP) and the calcium binding protein calmodulin to generate cAMP. The cAMP is measured by any of several known available methods. To facilitate diagnosis, a kit is provided containing the materials necessary for collection of secretion specimens, for storage of specimens pending assay, and transport of specimens to the assay laboratory, and for performing the assay and determining the result.
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Confer Dennis L.
Eaton John W.
Kepplinger Esther M.
Krawczewicz L.
Regents of the University of Minnesota
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