Method for the directional cloning of DNA

Details Method for the directional cloning of DNA Method for the directional cloning of DNA

1993-06-16

1996-06-04

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 536 2711, C12N 1511, C12N 1566

Patent

active

055232213

ABSTRACT:
A method for directionally cloning an insert DNA fragment into a target sequence using differential phosphorylation is disclosed, Monophosphorylated PCR fragments are directionally cloned into a monophosphorylated plasmid, Methods for directionally cloning non-PCR fragments into target DNA sequences are also discussed.

REFERENCES:
Sambrook, J. et al. (1989). Molecular Cloning: A Laboratory Manual, 2nd Ed., Cas, N.Y.
Kuisper, J. L. et al. (1992), Gene. 112(2) 147-155.

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