Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2011-06-07
2011-06-07
Nickol, Gary B. (Department: 1645)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007100
Reexamination Certificate
active
07955796
ABSTRACT:
Described is a method for the detection and quantification of methicillin-resistantStaphylococcus aureusfrom a specimen comprising a) contacting the specimen with Anti-Protein A antibodies so as to adsorb methicillinresistantStaphylococcus aureusand/or methicillin-sensitiveStaphylococcus aureus, b) separating said antibodies to which methicillin-resistantStaphylococcus aureusand/or methicillin-sensitiveStaphylococcus aureushave been adsorbed from the specimen, c) lysing methicillin-resistantStaphylococcus aureusand/or methicillin-sensitiveStaphylococcus aureusadsorbed to said antibodies so as to release their DNA, d) combining the released DNA with (i) probes and/or primers which are specific for a target DNA sequence of the mecA gene of methicillin -resistantStaphylococcus aureusand/or of methicillin-resistantStaphylococcusepidermis, (ii) probes and/or primers which are specific for a target DNA sequence of methicillin-resistantStaphylococcus aureusother than a target DNA sequence of the mecA gene and (iii) probes and/or primers which are specific for a target DNA sequence of methicillin-resistantStaphylococcusepidermis other than a target DNA sequence of the mecA gene, whereby the target DNA sequences of methicillin-resistantStaphylococcus aureusand of methicillin-resistantStaphylococcusepidermis are not homologous, e) subjecting the combined released DNA and the specific probes and/or primers to conditions which permit amplification of said target DNA sequences, f) detecting the presence and amount of the amplified target DNA sequences as an indication of the presence and amount of methicillin-resistantStaphylococcus aureus, new primers and probes and diagnostic kits for the detection and quantification of MRSA.
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BLAST alignment for Y14051, Aug. 13, 2009.
BLAST alignment for U23713, Aug. 13, 2009.
BLAST alignment for X17688, Aug. 13, 2009.
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Francois Patrice
Schrenzel Jacques
Gangle Brian J
Kratz Quintos & Hanson, LLP
Nickol Gary B.
Schrenzel Jacques
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