Method for the diagnosis of Helicobacter pylori infection,...

Chemistry: analytical and immunological testing – Testing of catalyst

Reexamination Certificate

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C435S007920, C514S588000

Reexamination Certificate

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07033838

ABSTRACT:
The method for the diagnosis ofHelicobacter pyloriinfection by the oral administration of defined amounts of13C-labeled urea and examination for13C content of blood samples removed at a defined time is effected bya) removing from 0.1 to 0.6 ml of capillary blood from the finger or ear lob of a patient or venous blood of a patient, in both cases with an empty stomach before the beginning of the test;b) administering an exact amount of from 10 to 50 mg of13C-urea in aqueous solution with a pH value of 2 to 4 to the patient;c) again removing capillary or venous blood exactly after 10 to 15 min from the administration; andd) determining the13C content of the blood samples by isotope ratio mass spectrometry (IRMS), and deducing the presence ofHelicobacter pylorifrom the increase of the13C values.

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patent: 6113875 (2000-09-01), Nyström et al.
patent: 6171811 (2001-01-01), Becerro De Bengoa Vallejo
patent: 296 13 373 (1996-11-01), None
patent: 0 881 491 (1998-12-01), None
Chey et al.“The13C-Urea Blood Test Accurately Detects Active Helicobacter pylori Infection: A United States, Multicenter Trial”, American Journal of Gastroenterology,94, No. 6 (1999), 1522-1524.
BIOSIS Abstract 2000: 5261.80;Alimentary Pharmocology&Therapeutics, 14,Supp. 3, 12-22 (2000) (Vaira et al.).
Cutler, A.F. and Toskes, P., MD, “Comparison of [13C] Urea Blood Test to [13C] Urea Breath Test for the Diagnosis of Heliobacter pylori”, American Journal of Gasterology, 94,No. 4 (1999), 959-61.
EMBASE Abstract 1999197569, American Journal of Gastroenterology, 94, 1522-1524(1999) (Chey et al.).

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