Method for the determination of unbound hormones and pharmaceuti

Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure

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23230B, 424 12, B01N 3316, G21H 502

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active

042358655

ABSTRACT:
Disclosed is a method for the determination of unbound hormones and pharmaceuticals comprising placing a solution containing unbound hormones, hormones bound to bonding proteins and bonding proteins into contact with an immobilized antibody; reacting the unbound hormone with the antibody; eluting the hormones bound to bonding proteins and the bonding hormones with a solution containing a marked hormone; reacting the marked hormone with the antibody; eluting the marked hormone not reacted with the antibody, and determining the marked hormone by radioimmunological evaluation.

REFERENCES:
patent: 3966897 (1976-06-01), Renn et al.
patent: 4039652 (1977-08-01), Adams et al.
patent: 4061466 (1977-12-01), Sjoholm et al.
Goodfriend et al., Immunochemistry, vol. 6, May, 1969, pp. 481-484.

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