Method for the determination of the presence of free light chain

Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase

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436539, 436501, 436512, C01N 33536

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active

051418770

ABSTRACT:
A method for the determination of the presence of free light chains (Bence Jones protein) in a unconcentrated and undiluted urine sample is provided in which the sample is reacted with an anti-free light chain antiserum reagent, where the presence of the free light chains is revealed by increase in turbidity of the reacted sample. By comparison with the turbidity of calibrators having predetermined concentrations reacted with anti-free light chain antiserum, a quantitative analysis of the amount of free light chains in the urine sample can be determined. A kit for performance of the analysis, including anti-free light chain antiserum reagent, calibrator, and reagent without antiserum, is also provided.

REFERENCES:
patent: 4211530 (1980-07-01), Goverde et al.
patent: 4311788 (1982-01-01), Heuck
patent: 4708939 (1987-11-01), Siedel et al.
patent: 4720465 (1988-01-01), Jensen et al.
patent: 4792529 (1988-12-01), Rudick et al.
patent: 4843021 (1989-06-01), Noguchi et al.
Heino, J.; Rajamaki, A.; Irjalak, "Turbidimetric measurement of Bence-Jones proteins using antibodies against free light chains of immunoglobulins. An artifact caused by different polymeric forms of light chains", Scand J. Clin. Lab Invest. Apr. 1984, 44(2) pp. 173-176 see abstract Dialog A.N.=84196104.

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