Method for the determination of the fat content of samples, pref

Chemistry: analytical and immunological testing – Lipids – triglycerides – cholesterol – or lipoproteins

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436 20, 436 23, 436154, 436161, 436171, 436174, 436175, 436177, 436178, G01N 3392

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056656025

ABSTRACT:
In a method for the determination of the fat content of samples, preferably organic samples, fat portions are dissolved out of the sample by means of extraction. A high boiling point solvent is used for extraction. Through the use of a high boiling point solvent, all possible fat portions will be dissolved out and the extraction time will be significantly reduced. Concurrent with extraction, the extracted fat portions are saponified through the addition of a base, as a result of which salts of the fatty acids contained in the sample will be formed. In further procedural steps, these salts of the fatty acids are subjected to further treatment, separated from one another and analysed. The method enables the exact determination of the fat content of organic samples on the basis of the fatty acids contained within the sample.

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The Condensed Chemical Dictionary, 10th Edition, Hawley, 1981, p. 909.

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