Method for the determination of the concentration of an enzyme s

Details Method for the determination of the concentration of an enzyme s Method for the determination of the concentration of an enzyme s

1993-03-29

1994-08-23

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 25, 435 26, 435 28, C12Q 154, C12Q 126, C12Q 132, C12Q 128

Patent

active

053407229

ABSTRACT:
A method for the continuous and reversible determination of the concentration of an enzyme substrate such as glucose in an specimen, wherein the specimen is brought into contact with a corresponding enzyme selected from oxidases and oxygenases and to which a flavin coenzyme (FMN, FAD) is bonded, the flavin coenzyme changing to a reduced form by the enzyme substrate and to an oxidized form by molecular oxygen dissolved in the specimen, and the change in the fluorescence spectrum, produced by means of the reduction of the flavin coenzyme, is measured.

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Chemical Abstracts, vol. 95, p. 397 (1981) 95:765093.
Wangsa et al., "Fiber-Optic Biosensors Based on the Fluorometric Detection of Reduced Nicotinanmide Adenine Dinucleotide" in Anal. Chem. 60, 1080 (1988).

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