Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor
Patent
1988-07-01
1991-10-15
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving blood clotting factor
435 23, C12Q 156
Patent
active
050574140
ABSTRACT:
The invention relates to a method for the determination of the activity of serine proteases and serine protease inhibitors in plasma or other biological fluids.
The only methods hitherto available for rapid and accurate determination of the content of serine proteases or serine protease inhibitors have been very time-consuming and methodologically demanding. In the method according to the invention for the determination of the activity of serine proteases or serine protease inhibitors, specific inhibitors are inactivated by addition of at least one oxidizing agent and, where appropriate, at least one detergent. The addition of an omega-aminocarboxylic acid enhances and observed effects when the serine protease is plasmin.
REFERENCES:
patent: 4493891 (1985-01-01), Travis
patent: 4818690 (1989-04-01), Paques
Beatty et al, "Kinetics of Association of Serine . . . ", Journal of Biological Chemistry, 255:3931-3934 (1980).
Verheijen et al, "Plasminogen Effectors", Methods of Enzymatic Analysis, vol. V, pp. 425-433 (1984).
Chemical Abstracts, vol. 96, No. 19, May 10, 1982, p. 517, Abstract 160021t, Allen, R. A., "An Enhancing Effect of . . . ".
Ichinose et al, "The Activation of Pro-Urokinase by . . . ", Journal of Biological Chemistry, 261:3486-3489 (1986).
Kluft et al, "Appropriate Millieu for the Assay . . . ", Haemostasis, 15:198-203 (1985).
Leung et al, "Poly-D-Lysine Dependent Inactivation . . . ", Thrombosis Research, 46:767-777 (1987).
Speiser et al, "Method for the Determination of Fast Acting Plasminogen Activator Inhibitor Capacity (PAI-CAP) in Plasma, Platelets and Endothelial Cell," Thrombosis Research, 44:503-515, 1986.
Chmielewska et al, Determination of Tissue Plasminogen Activator and Its "Fast" Inhibitor in Plasma, Clinical Chemistry, 32; 3:482-485, 1986.
Lawrence et al, Inactivation of Plasminogen Activator Inhibitor by Oxidants, Biochemistry 25; 21:6351-6355, 1986.
Shieh et al, The Reactive Site of Human .alpha..sub.2 -Antiplasmin, Journal of Biological Chemistry, 262; 13:6055-6059, 1987.
Shechter et al, Selective Oxidation of Methionine Residues in Proteins, Biochemistry, 14; 20:4497-4503, 1975.
Weiss et al, Long-Lived Oxidants Generated by Human Neutrophils: Characterization and Bioactivity, Science 222; Nov. 11, 1983, pp. 625-628.
Heimburger Norbert
Stief Thomas
Behringwerke Aktiengesellschaft
Rosen Sam
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