Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1986-01-30
1988-10-18
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 11, 435 14, 435 15, 435 16, 435 18, 435 19, 435 22, 435 24, 435 25, C12Q 128
Patent
active
047787570
ABSTRACT:
There is provided an improved quantitative analysis for the determination of a substrate or enzyme. In the analysis for the enzyme, it is reacted with a known substrate and in the analysis for the substrate, it is reacted with a known enzyme and then the resultant substance is reacted with an oxidase to produce hydrogen peroxide which is then reacted with a peroxidase, phenol derivatie and coupler to form a colored material which is then subjected to colorimetic analysis. The improvement resides in conducting the foregoing process by adding to the test sample a first reagent containing the oxidase, peroxidase and phenol derivative represented by the formula: ##STR1## wherein R.sub.1 is .circle.a a lower alkyl group having one to five carbon atoms or .circle.b the group defined in .circle.a above having a hydroxyl or sulfonic acid group, R.sub.2 is a hydrogen or halogen atom, a lower alkyl group having one to five carbon atoms, a lower acyl group having one to five carbon atoms, a lower alkylether group having one to five carbon atoms, or a lower alkoxycarbonyl group having one to five carbon atoms, said groups being unsubstituted or substituted with a hydroxyl or sulfonic acid group, and n is 0 to 4. Then, there is added to the above mixture a second reagent containing the coupler and the enzyme.
REFERENCES:
patent: 4416982 (1983-11-01), Tsuda et al.
Mitsuhida Noboru
Nakagiri Yoshitaka
Teshima Shin'ichi
Rosen Sam
Toyo Boseki Kabushiki Kaisha
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