Method for the determination of substrates or enzyme activities

Details Method for the determination of substrates or enzyme activities Method for the determination of substrates or enzyme activities

1977-04-28

1979-09-18

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving uric acid

435 14, 435 25, 435 28, G01N 3316

Patent

active

041682050

ABSTRACT:
In a process for the determination of substrates or enzyme activities in a test batch, utilizing a conventional redox reaction as a measurement reaction, the improvement comprising carrying out the process in the presence of ascorbate oxidase; the addition of ascorbate oxidase substantially eliminates the interfering effect of ascorbic acid frequently found in the test sample.

REFERENCES:
patent: 3721607 (1973-03-01), Gruber et al.
patent: 3791988 (1974-02-01), Josef et al.
patent: 3992158 (1976-11-01), Przybylowicz et al.
Gerwin et al., Journal of Biological Chemistry, vol. 249, No. 7, pp. 2005-2008, Apr. 1974.
Bailey, The Standard Cyclopedia of Horticulture, pp. 909, 910, 2860 and 2861 (1943).

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