Method for the determination of small substance quantities of me

Details Method for the determination of small substance quantities of me Method for the determination of small substance quantities of me

1986-01-10

1992-06-23

Saunders, David

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

250282, 436161, 436173, 436817, G01N 33543

Patent

active

051242672

ABSTRACT:
The invention relates to a process for the determination of small substance quantities of medicines, of endogenous or other chemical compounds in biological material, characterized by either binding the compound to be determined and its deuterated analog to an antibody present in excess, which antibody is coupled to a stationary phase of a polymeric material, and separating the two compounds from the antibody and measuring by mass spectrometry the proportion of deuterated compound
ot deuterated compound or, respectively, cross-reacting compound; or, in case a suitable deuterated or cross-reacting compound is not available, utilizing as the internal standard also a chemically similar compound which, however, is added after the antibody-mediated extraction, and inserting a separating process before mass spectrometry.

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