Method for the determination of mutant restriction enzymes

Details Method for the determination of mutant restriction enzymes Method for the determination of mutant restriction enzymes

1997-07-16

1999-09-21

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 18, C12Q 134

Patent

active

059553693

ABSTRACT:
The present invention is directed to a method for the determination of mutant restriction enzymes which comprises incubating restriction enzymes under non-native conditions with a labeled double stranded oligonucleotide to a solid support to form an enzyme-oligonucleotide complex and detecting the label to determine cleavage of the oligonucleotide by mutant enzymes.

REFERENCES:
patent: 4775619 (1988-10-01), Urdea
Yebra, M. J., et al. (1993) Nuc. Acids Res. 21(24), 5797-5798.
Bischofberger, N, et al. (1987) Nuc. Acids Res. 15(2), 709-716.
Estrada, G., et. al. (1996) Mol. Cell. Probes 10, 179-185.
Jsnulaitsi, A. A., et al. (1981) Anal. Biochem. 116, 116-122.

Affiliated with

Also associated with

Rating

Method for the determination of mutant restriction enzymes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the determination of mutant restriction enzymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the determination of mutant restriction enzymes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-79815