Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1990-12-21
1992-07-28
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 30, 435 34, 435 29, 435297, 435299, 435300, 435301, C12Q 106, C12Q 124, C12M 116
Patent
active
051340640
ABSTRACT:
The invention relates to a method for the determination of microbe concentrations by means of a plating method, wherein a sample and a medium are plated in a dish, the medium is allowed to solidify and the solidified plate is incubated, wherein the colonies in the plate are counted. According to the method the microbes are inoculated to a medium solidifying in a dish and in such a manner that its layer thickness varies in a controlled manner. Also disclosed is a dish which is suitable for the determination of microbe concentrations by means of a plating method and the bottom part of which has such a shape that a solidified layer is formed in the dish after the plating of the sample and the medium, the thickness of the layer varying in a controlled manner.
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Thomson, Donnelly & Black 1960 A Plate Loop Method for Determining Viable Counts of Raw Milk, J. Milk Food Technol. 26: 156 to 171 cited in the Specification.
FIL-IDF 100:1981 Liquid Milk Enumeration of Microorganisms Colony Count Technique at 30 C. cited in the Specification p. 11. Corresponding to ISO/DP 6610.
Standard Methods for the Examination of Dairy Products, pp. 77-93; 315-317 (E. H. Marth ed. 14th ed. 1978).
Labinskaya, A. S. Microbiology and Technical Means For Carrying Out Microbiological Studies, Medicine Publishers, Moscow, pp. 52-53 (1968).
Yegorov, N. S., Antagonist Microbes and Biological Methods For the Determination of Antibiotic Activity, Uysslaya Shkola Pub., Moscow, pp. 51-54 (1965).
Kepplinger Esther L.
Scheiner Toni R.
Valio Meijerien Keskusosuusliike
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