Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1996-05-09
1998-06-02
Leary, Louise
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, 435 26, 435 4, 435 14, 435817, 4352871, 4352875, 562589, 204403, 204297, C12Q 128, C12Q 126, C12Q 100
Patent
active
057597960
ABSTRACT:
A method is described for the determination of lactic acid in organic materials of alimentary interest that involves reacting, in a buffered aqueous medium, a sample of the said materials, or a solution obtained by extracting them, with an enzyme system comprising L(+) lactate oxidase, D(-) lactate dehydrogenase and horseradish peroxidase and measuring the concentration of any oxygen produced as a result of the oxidation of the lactic acid contained in the said sample with an amperometric electrode that is selective for oxygen.
A biosensor that can be used to put the above method into effect is also described.
REFERENCES:
patent: 4166763 (1979-09-01), Esders et al.
patent: 4237222 (1980-12-01), Misaki et al.
patent: 4592996 (1986-06-01), Yamanishi et al.
Cheng et al, Clinica Chimica Acta, vol. 91, pp. 295-301, (1979). Month not available.
Azzoni Alfredo
Belicchi Corrado
Botre Claudio
Cavalieri Barbara
Mazzei Franco
CO.RI.AL. S.C.P.A.
Leary Louise
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