Method for the determination of lactic acid in organic materials

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 25, 435 26, 435 4, 435 14, 435817, 4352871, 4352875, 562589, 204403, 204297, C12Q 128, C12Q 126, C12Q 100

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active

057597960

ABSTRACT:
A method is described for the determination of lactic acid in organic materials of alimentary interest that involves reacting, in a buffered aqueous medium, a sample of the said materials, or a solution obtained by extracting them, with an enzyme system comprising L(+) lactate oxidase, D(-) lactate dehydrogenase and horseradish peroxidase and measuring the concentration of any oxygen produced as a result of the oxidation of the lactic acid contained in the said sample with an amperometric electrode that is selective for oxygen.
A biosensor that can be used to put the above method into effect is also described.

REFERENCES:
patent: 4166763 (1979-09-01), Esders et al.
patent: 4237222 (1980-12-01), Misaki et al.
patent: 4592996 (1986-06-01), Yamanishi et al.
Cheng et al, Clinica Chimica Acta, vol. 91, pp. 295-301, (1979). Month not available.

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