Method for the determination of hemoglobin adducts

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 21, 435 25, 435 26, 436 15, 436 66, 436 67, 436518, 436534, 422 681, 600328, G01N 3353, G01N 3100, G01N 3372, G01N 1506

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060430435

ABSTRACT:
Disclosed is an improvement to the method of determining the concentration of a hemoglobin adduct in a blood sample by the steps of assaying the blood sample for the total amount of hemoglobin, assaying the blood sample for the hemoglobin adduct, and dividing the hemoglobin adduct concentration by the total hemoglobin concentration. The improvement involves normalizing the measurement of the hemoglobin adduct to the total amount of hemoglobin in the blood sample.

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patent: 4970171 (1990-11-01), Messenger et al.
Little et al Clin. Chem. 32/2, pp. 358-360(1986) "Interlaboratory Standardization of Glycoted Hemoglobin Determinants".
Standing et al Ann. Clin. Biochem. 29 494-505 (1992) "Glycoted Haemoglobin; an Assessment of High Capacity Liquid Chromatographic & Immunoassay Methods,".
Morrero et al Diabetes Care 15 #8 pp. 1045-1049 (Aug. 1992) "Immediate HbAic Results".
Rodgers in Practical Immunoassay ed-Butt, Marcel Dekker Inc. (1984)pp. 253-307 "Data Analysis and Quality Control of Assays! A Practical Primer".

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