Method for the determination of glycosylated proteins

Details Method for the determination of glycosylated proteins Method for the determination of glycosylated proteins

1998-11-19

1999-11-16

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 23, 435 4, C12Q 128, C12Q 137, C12Q 100

Patent

active

059855916

ABSTRACT:
A method for measuring the amount of glycosylated protein comprising the steps of decomposing glycosylated proteins with protease, causing a redox reaction between the decomposed products and an Amadori compound oxidoreductase, and determining the redox reaction so as to measure the amount of glycosylated proteins, wherein the decomposition with the protease is carried out in the presence of at least one substrate selected from the group consisting of metalloporphyrin, cytochrome and diaphorase.

REFERENCES:
patent: 5370990 (1994-12-01), Staniford et al.
patent: 5639672 (1997-06-01), Burd et al.
The Journal of Biological Chemistry vol. 239, No. 11, Nov. 1964, p. 3790-3796, Sangduk Kim et al., "Purification and Properties of the Enzyme" .

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