Textiles: fiber preparation
Patent
1976-09-13
1978-03-21
Shapiro, Lionel M.
Textiles: fiber preparation
G01N 3114
Patent
active
040802655
ABSTRACT:
A method for the detection and the quantitative determination of the enzyme creatine phosphokinase (CPK), an agent present in abnormal concentrational levels in serum in many cases of mental disorders and neuromuscular disorders, and significant in the diagnosis of myocardial infarction. The component such as a blood-impregnated carrier substance, obviating the need for separatory procedures (i.e., separating the serum from the blood cells) and special handling (i.e. freezing, freeze drying, etc.) to preserve the sample. The mechanism of the laboratory determination is that adenosine diphosphate (ADP) reacts with creatine phosphate in the presence of creatine phosphokinase and magnesium ions to produce adenosine triphosphate (ATP) plus creatine. The ATP, in turn, reacts with the luciferin and luciferase of the firefly lantern system to produce light (bioluminescence), the intensity of which is proportional to the concentration of CPK initially present. There is provided a test reagent system for carrying out the method of the invention.
REFERENCES:
patent: 3838012 (1974-09-01), Higgens et al.
Bergmeyer's "Methods of Enzymatic Analysis", vol. 1 (1974) Academic Press, Inc., N.Y., pp. 165-171.
Strehler et al., Arch. Biochem. Biophysics, 40 (1952) pp. 28-41.
Berkman Michael G.
Fan C. A.
Shapiro Lionel M.
LandOfFree
Method for the determination of creative phosphokinase enzyme does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the determination of creative phosphokinase enzyme, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the determination of creative phosphokinase enzyme will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-569792