Method for the determination of creatine phosphokinase enzyme

Details Method for the determination of creatine phosphokinase enzyme Method for the determination of creatine phosphokinase enzyme

1974-08-02

1977-01-04

Monacell, A. Louis

Textiles: fiber preparation

195 99, C12K 104, G01N 3300

Patent

active

040010880

ABSTRACT:
A method for the detection and the quantitative determination of the enzyme creatine phosphokinase (CPK), an agent present in abnormal concentrational levels in serum in many cases of mental disorders and neuromuscular disorders, and significant in the diagnosis of myocardial infarction. The method utilizes a dried time-stable sample of a pure or mixed component such as a blood-impregnated carrier substance, obviating the need for separatory procedures (i.e., separating the serum from the blood cells) and special handling (i.e. freezing, freeze drying, etc.) to preserve the sample. The mechanism of the laboratory determination is that adenosine diphosphate (ADP) reacts with creatine phosphate in the presence of creatine phosphokinase and magnesium ions to produce adenosine triphosphate (ATP) plus creatine. The ATP, in turn, reacts with the luciferin and luciferase of the firefly lantern system to produce light (bioluminescence), the intensity of which is proportional to the concentration of CPK initially present. There is provided a test reagent system for carrying out the method of the invention.

REFERENCES:
patent: 3403077 (1965-09-01), Beyer et al.
patent: 3485724 (1969-12-01), Beyer et al.
B. L. Strehler et al., "Firefly Luminescence in the Study of Energy Transfer Mechanisms, I. Substrate and Enzyme Determination", Arch Biochem. Biophysics 40, pp. 28-41, 1952.
H. U. Bergmeyer "Methods of Enzymatic Analysis", pp. 559-572, Academic Press, N.Y. and London, 1965.
Dalal et al., Clin. Chem., vol. 18, No. 4, pp. 330-334, 1972.

Affiliated with

Also associated with

Rating

Method for the determination of creatine phosphokinase enzyme does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the determination of creatine phosphokinase enzyme, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the determination of creatine phosphokinase enzyme will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-251485