Method for the determination of bilirubin in solution

Details Method for the determination of bilirubin in solution Method for the determination of bilirubin in solution

1988-01-28

1990-06-26

Deck, Randall E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 25, C12Q 128, C12Q 126

Patent

active

049371860

ABSTRACT:
A novel analytical method for bilirubin in biological fluids. Bilirubin is catalytically oxidized to biliverdin in the presence of bilirubin oxidase (BOX) and the latter is revertedly back-converted to initial bilirubin in the presence of suitable reducing agents. The corresponding oxidation of said reducing agents provides a signal which is monitored and provides the desired analytical data.
When using selected organic fluoro-compound, said signal is provided by the release of F.sup.- ions which can be measured electrometrically.

REFERENCES:
patent: 4211844 (1980-07-01), Wu
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patent: 4517287 (1985-05-01), Scheibe et al.
patent: 4571383 (1986-02-01), Takayama et al.
patent: 4600689 (1986-07-01), Matsui et al.
patent: 4607010 (1986-08-01), Siddiqi et al.
patent: 4701411 (1987-10-01), Wu
patent: 4781890 (1988-11-01), Arai et al.

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