Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1992-08-05
1993-11-23
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435856, C12Q 132
Patent
active
052643476
ABSTRACT:
The selective determination of .alpha.-ketoisocaproate in samples of body fluids, especially in serum, is achieved, without previous separation, directly in the fluid and in the presence of NADH, by means of a KIC-specific D-.alpha.-hydroxy-isocaproic dehydrogenase that originates specifically from strains of Lactobacillus casei producing this enzyme, in particular strains of Lactobacillus casei subspecies pseudoplantarum or casei. The KIC determination is used, in particular, for diagnosing maple syrup urine disease. Expedient for this purpose are analytical kits which have a divisible stock of D-.alpha.-hydroxyisocaproic dehydrogenase from Lactobacillus casei, in particular from the Lactobacillus casei (DSM 20008).
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Hummel Werner
Schadewaldt Peter
Wendel Udo
Kernforschungsanlage Juelich GmbH
Naff David M.
Saucier Sandra
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