Method for the determination of .alpha.-amylase

Details Method for the determination of .alpha.-amylase Method for the determination of .alpha.-amylase

1979-03-19

1981-05-19

Hruskoci, Peter A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435805, 536 45, C12Q 140

Patent

active

042686288

ABSTRACT:
Novel method for the determination of .alpha.-amylase which method comprises contacting a sample suspected of containing .alpha.-amylase with a starch derivative carrying a substituent capable of dyestuff-forming coupling; separating from the resulting starch-containing phase the low molecular weight soluble fission products formed by splitting of said starch derivative by .alpha.-amylase; coupling the said couplable substituent with another dyestuff forming component; and measuring the dyestuff so formed as a measure of the initial .alpha.-amylase content.

REFERENCES:
patent: 2317752 (1943-04-01), Fuller
patent: 3117105 (1964-01-01), Borchert
patent: 3219518 (1965-11-01), Barber et al.
patent: 3679661 (1972-07-01), Babson
patent: 3694318 (1972-09-01), Klein et al.
patent: 3888739 (1975-06-01), Whetzel et al.
patent: 4025392 (1977-05-01), Dougherty
patent: 4144306 (1979-03-01), Figueras

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