Chemistry: analytical and immunological testing – Lipids – triglycerides – cholesterol – or lipoproteins
Reexamination Certificate
2008-04-15
2008-04-15
Wallenhorst, Maureen M. (Department: 1797)
Chemistry: analytical and immunological testing
Lipids, triglycerides, cholesterol, or lipoproteins
C436S020000, C436S161000, C436S173000, C436S174000, C436S177000, C436S178000, C210S656000
Reexamination Certificate
active
10581789
ABSTRACT:
A method for quantitative determination of 25-hydroxycholecalciferol in feed is described. The method includes the steps of adding a defined amount of an internal standard which has a mass different from 25-hydroxycholecalciferol and a polarity similar to that compound, e.g., 26,27-hexadeutero-25-hydroxycholecalciferol, to an aqueous dispersion of the feed, extracting the aqueous dispersion with tert.butyl methyl ether and further processing the extract by HPLC and mass spectrometry as described in the specification.
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International Search Report.
Denu Laurent
Goessl Richard
Hofmann Peter
DSM IP Assets B.V.
Nixon & Vanderhye P.C.
Wallenhorst Maureen M.
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