Method for the detection of target nucleic acid

Details Method for the detection of target nucleic acid Method for the detection of target nucleic acid

1995-07-17

1998-11-03

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 5, 435 911, 536 243, 536 2432, 536 2433, 536 231, C12Q 168, C12P 1934, C07H 2104, C07H 2102

Patent

active

058306641

ABSTRACT:
Method for the sensitive detection of a target nucleic acid by hybridization with a probe nucleic acid. The latter contains a part which can hybridize with the target nucleic acid and a nucleic acid-specific part which does not hybridize with the target nucleic acid. The method further comprises cleavage of the probe nucleic acid, hybridization of a cleavage product of the probe nucleic acid containing the part that does not hybridize with the target nucleic acid with a matrix nucleic acid containing a part that can be hybridized with the cleavage product and a part that cannot be hybridized with the probe nucleic acid. The method also comprises the determination of the hybrid consisting of the cleavage product and the matrix nucleic acid and a reagent kit suitable for this purpose.

REFERENCES:
patent: 5200314 (1993-04-01), Urdea
patent: 5232829 (1993-08-01), Longiaru et al.
patent: 5422253 (1995-06-01), Dahlberg et al.
patent: 5518901 (1996-05-01), Murtagh
patent: 5573914 (1996-11-01), Love
PCT International Publication No. WO 91/17264 published Nov. 14, 1991.
BioTechniques, "Probe Amplifier System Based on Chimeric Cycling Oligonucleotides", vol. 9, No. 2 (1990), pp. 142-147.

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