Method for the detection of micrometastases in mesenchymal tissu

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 724, 435 792, 436 63, 436 64, 436813, G01N 33574, G01N 3353, G01N 33543

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active

053994825

ABSTRACT:
The present invention concerns a method for the detection of micrometastases of ectodermal or entodermal tumors. Cells from mesenchymal tissue, preferably from bone marrow, are subjected to a fractional lysis. Cytokeratins or fragments thereof are immunologically detected in the cell supernatant.

REFERENCES:
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patent: 5168044 (1992-12-01), Joyce et al.
Abstract of SU 1 649 444, Derwent Publications Ltd. Database WPI, Section Ch, Week 9217.
Bergmeyer, Methods of Enzymatic Analysis, 3rd Edition, vol. II, 1983, p. 33, table 2.
Schlimok et al., "Micrometastatic Tumor Cells in Bone Marrow of Patients with Gastric Cancer: Methodological Aspects of Detection and Prognostic Significance," Eur J Cancer 27(11) 1461-65 1991.
Hall et al., "Characterization of the Intermediate Filament Proteins of Murine Mammary Gland Epithelial Cells," Experimental Cell Research, 162; pp. 379-389, 1986.

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