Radiant energy – Invisible radiant energy responsive electric signalling – Infrared responsive
Patent
1995-11-13
2000-02-29
Smith, Ruth S.
Radiant energy
Invisible radiant energy responsive electric signalling
Infrared responsive
128664, 25033912, G01N 2135
Patent
active
060312327
ABSTRACT:
This invention discloses a method to identify premalignant and malignant stages of cervical cancer from an infrared (IR) spectrum of exfoliated cervical cells which are dried on an infrared transparent matrix and scanned at the frequency range from 3000-950 cm.sup.-1. The identification of samples is based on establishing a calibration using a representative set of spectra of normal, dysplastic and malignant specimens. During the calibration process, multivariate techniques such as Principal Component Analysis (PCA) and/or Partial Least Squares (PLS) are used. PCA and PLS reduce the data based on maximum variations between the spectra, and generate clusters in a multidimensional space representing the different populations. The utilization of Mahalinobis distances, or linear regression (e.g., Principle Component Regression on the reduced data from PCA) form the basis for the discrimination. This method is simple to use and achieves statistically reliable distinction between the following groups of cervical smears: normal (individuals with no prior history of dysplasia), dysplasia and malignant samples. Lastly, this invention discloses a method to obtain the IR spectrum of individual cervical cells fixed on an infrared transparent matrix.
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Bhandare Prashant S.
Cahn Frederick R.
Cohenford Menashi A.
Krishnan Krishnaswamy
Rigas Basil
Bio-Rad Laboratories, Inc.
Smith Ruth S.
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