Method for the detection of lysozyme using a protein error indic

Details Method for the detection of lysozyme using a protein error indic Method for the detection of lysozyme using a protein error indic

1996-09-03

1998-05-19

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 28, 436904, C12Q 134, C12Q 128

Patent

active

057534551

ABSTRACT:
The determination of lysozyme in urine is carried out by contacting the urine with a reagent system containing a buffer and a protein error indicator dye. Other proteins normally found in urine can compete with the lysozyme for interaction with the protein error indicator thereby affecting the specificity of the test for lysozyme. The present invention involves the addition of certain alkyl sulfonic acids or sulfonates to increase both specificity and sensitivity of the reagent for lysozyme.

REFERENCES:
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Kragh-Hansen U., Relation Between Binding of Phenolsulfophthalein Dyes and Other Ligands With a High Affinity for Human Serum Albumin. Biochimica et Biophysica Acta 365:360-371, 1974.
Y. Fujita, Bunseki Kagaku, vol. 32, pp. E379-E386, 1983 "Color Reaction Between Pyrogallol Red--Molybdenum (VI) Complex and Protein".
Kragh-Hansen et al., Biophysics Acta, (365), pp. 360-371 (1974) Protein Binding of Small Molecules.
Macart et al., Clinica Chimica Acta, (141). pp. 77-84 (1984) Evaluation of an Improved Coomassie Dye Binding Method for Urinary Protein Assay.

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