Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-02-17
1997-02-18
Budens, Robert D.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 71, 5303881, 5303883, 5303889, C12Q 170, G01N 3353, C07K 1600, C12P 2108
Patent
active
056040920
ABSTRACT:
Monoclonal antibodies to cyclosporine A (CsA), a potent immunosuppressant, were generated in Balb/c mice using a novel antigen prepared by linking CsA to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-CsA antibodies were generated, using CsA-BBa-bovine serum albumin (CsA-BBa-BSA) as the immunogen. Epitope mapping studies were performed using a series of singly substituted CsA derivatives and an antibody was identified that recognizes N-methyl leucine residues 9 and 10 of CsA. This antibody also cross-reacts with the HIV-1 Gag protein. A method for the detection of HIV-1 in patient samples employing this monoclonal antibody is disclosed.
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Chen Bi-Xing
Erlanger Bernard F.
Budens Robert D.
Parkin Jeffrey S.
The Trustees of Columbia University in the City of New York
White John P.
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