Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-08-06
1998-12-08
Sisson, Bradley L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 536 231, 536 235, 536 2433, 935 76, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500
Patent
active
058467106
ABSTRACT:
Method for screening a sample oligonucleotide for a variation in sequence at a predetermined position thereof relative to a nucleic acid the sequence of which is known, wherein the sample oligonucleotide is provided as a single stranded molecule, the single stranded molecule is mixed with an inducing agent, a labeled nucleotide, and a primer having a sequence identical to a region flanking the predetermined position to form a mixture, the mixture having an essential absence of nucleotides constituted of bases other than the base of which the labeled nucleotide is constituted, the mixture is subjected to conditions conducive for the annealing of the primer to the single stranded molecule and the formation of a primer extension product incorporating the labeled nucleotide, and the mixture is analyzed for the presence of primer extension product containing labeled nucleotide.
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Sisson Bradley L.
St. Louis University
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