Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2011-04-05
2011-04-05
Staples, Mark (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S024300
Reexamination Certificate
active
07919243
ABSTRACT:
A method for the detection ofFusarium graminearum(Gibberella zeae) comprising the steps: providing a sample containing a nucleic acid, contacting said sample with at least one forward primer and at least one reverse primer, wherein the at least one reverse primer hybridises within the β-tubulin nucleic acid sequence ofFusarium graminearum(Gibberella zeae) and comprises the nucleic acid sequence 5′-R1TTTTCGTX1GX2AGT-3′ (SEQ ID NO 1), wherein R1comprises at least one nucleic acid residue of the β-tubulin nucleic acid sequence located upstream of the hybridisation site of the nucleic acid sequence SEQ ID NO 1 and subsequent to the nucleic acid sequence SEQ ID NO 1, X1is guanine, adenine or inosine, X2is cytosine, adenine or inosine, and wherein the at least one forward primer hybridises upstream of the hybridisation site of a nucleic acid sequence complementary to the at least one reverse primer, subjecting the sample contacted with the at least one forward primer and the at least one reverse primer to a nucleic acid amplification technique, and optionally determining the presence ofFusarium graminearum(Gibberella zeae) in a sample by detecting a nucleic acid amplification product.
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Farnleitner Andreas
Mach Robert
Reischer Georg
Fulbright & Jaworski LLP
Staples Mark
Technische Universitat Wien
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