Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation
Reexamination Certificate
2006-10-10
2006-10-10
Toatley, Jr., Gregory J. (Department: 2877)
Optics: measuring and testing
By dispersed light spectroscopy
With sample excitation
Reexamination Certificate
active
07119898
ABSTRACT:
A method for the detection and evaluation of the light generated in a fluorescing specimen by a short pulse laser, wherein at least a first and a second fluorophore and/or a self-fluorescing specimen are separately irradiated with different wavelengths and the specimen light is recorded in a wavelength-dependent manner with at least one nondescanned detector as reference spectrum and a separation into individual spectra is carried out during the irradiation of at least two fluorophores and/or self-fluorescing specimens simultaneously from the measured spectrum and the reference spectra through regression analysis, wherein the wavelength of the short pulse laser is advantageously changed continuously in at least one wavelength region.
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Sensitive Imaging of Spectrally Overlapping Fluorochromes using the LSM 510 META, Mary E. Dickinson, et al. Multiphoton Microscopy in the Biomedical Sciences II, Ammasi Periasamy, Peter .C. So, Editors, vol. 4262 2002.
Dickinson Mary
Simbuerger Eva
Zimmermann Bernhard
Carl Zeiss Jena GmbH
Geisel Kara
Reed Smith LLP
Toatley , Jr. Gregory J.
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