Chemistry: analytical and immunological testing – Clotting or clotting factor level tests
Patent
1987-10-29
1991-05-14
Lacey, David L.
Chemistry: analytical and immunological testing
Clotting or clotting factor level tests
436175, 436178, 436528, 435 75, 435 13, G01N 33573
Patent
active
050155881
ABSTRACT:
An assay method for the detection of Factor XIII in plasma is disclosed in which a primary amine derivative of biotin such as preferably 5-(biotinamido) pentylamine is incubated with a glutamine substrate and activated Factor XIII (Factor XIIIa) to form a biotinylated product which may be measured by convention detection assays. In a preferred embodiment, the biotinylated product is bound to a well in a microtiter plate or other solid support and the product is measured by a colorimetric assay which may be read by an automated spectrophotometric plate reader.
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Birckbichler Paul J.
Lee Kyung
Patterson, Jr. Manford K.
Lacey David L.
Snay Jeffrey R.
The Samuel Roberts Noble Foundation, Inc.
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