Method for the detection of enzyme-catalyzed cleavage...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C435S024000, C435S004000

Reexamination Certificate

active

07425425

ABSTRACT:
A method involves the detection of enzyme-catalyzed cleavage reactions using modular chemical compounds as substrates for the enzymes involved, and detection is effected by use of molecular-weight-sensitive methods.

REFERENCES:
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patent: 2001/0046668 (2001-11-01), Levine et al.
patent: 2 791 141 (1999-03-01), None
patent: WO 00/52199 (2000-09-01), None
patent: WO 00/72016 (2000-11-01), None
Bergmann, F. and Bannwarth, W., “Solid Phase Synthesis of Directly Linked Peptide-Oligodeoxnucleotide Hybrids Using Standard Synthesis Protocols”, Tetrathedron Letters, 36:11 (1995), 1839-42.
Levine et al., “Measurement of Specific Protease Activity Utilizing Fluorescence Polarization”, Analytical Biochemistry, 247 (1997), 83-88.
Robles, J., “Synthesis and Enzymatic Stability of Phosphodiester-Linked Peptide-Oligonucleotide Hybrids”, Bioconjugate Chem., 8 (1997), 785-88.
Robles, J. et al., “Towards Nucleopeptides Containing Any Trifunctional Amino Acid”, Tetrahedron, 55 (1999), 13251-64.
Singh et al., “Fluorescence Polarization for Monitoring Ribozyme Reactions in Real Time”, Biotechniques, 29 (2000), 344-51.

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