Method for the detection of creatinine

Details Method for the detection of creatinine Method for the detection of creatinine

1996-06-17

1998-03-31

Wallenhorst, Maureen M.

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-n

436 80, 436164, 436169, 436904, 435 4, 435 28, 422 56, G01N 3370

Patent

active

057337870

ABSTRACT:
Disclosed is a method for the determination of creatinine in urine which involves contacting a urine test sample with a reagent system containing Cu.sup.+2, a hydroperoxide and an oxidizible dye which provides a detectable response when oxidized in the presence of a pseudoperoxide. The reagent system also includes a buffer to maintain the urine's pH within the range of 6.6 to 8.0 and a chelating agent for the cupric ions to prevent oxidation of the dye in the absence of creatinine.

REFERENCES:
patent: 5173431 (1992-12-01), Pugia et al.
patent: 5374561 (1994-12-01), Pugia
patent: 5385847 (1995-01-01), Yip et al.
patent: 5464777 (1995-11-01), Yip
patent: 5527708 (1996-06-01), Blass

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