Chemistry: analytical and immunological testing – Nitrogen containing
Patent
1991-12-18
1993-04-27
Johnston, Jill A.
Chemistry: analytical and immunological testing
Nitrogen containing
436104, 436120, 436172, G01N 2164, G01N 3122
Patent
active
052061752
ABSTRACT:
The process of detecting the presence on a surface of at least one of bis(2-chloroethyl)sulfide and bis(2-chloroethyl)imine, including (a) providing a solution comprising at least one dyestuff selected from the group consisting of brilliant blue R, guinea green B, neutral red, and lissamine green B, which at least one dyestuff is capable of fluorescing and is capable, when applied as a solution, of forming an adduct with at least one of bis(2-chloroethyl)sulfide and bis(2-chloroethyl)imine when present on a surface, which at least one adduct, is capable of fluorescing in a spectral range which is different from that of the corresponding at least one dyestuff; (b) applying the solution to the surface; (c) irradiating the surface onto which the solution has been applied with light having a wavelength effective to excite the at least one dyestuff and, when present, the at least one adduct, and induce light emission by fluorescence therefrom; (d) measuring the wavelength of the light emitted from the surface; and (e) determining the presence of the at least one adduct on the surface from the presence of a measured difference in the wavelength of the light emitted by the at least one adduct and the at least one dyestuff.
REFERENCES:
patent: 3960759 (1976-06-01), Mallis
patent: 4565787 (1986-01-01), Bossle et al.
CA-99:189045b (Appler et al).
CA-96:194568s (Fritsche et al).
CA-86:154765r-Wirth P. et al.
CA-96:199415t-Kramer et al.
Boscher Jorg
Diehl Wolfgang
Krieger Wolfram
Rossmann Klaus
Battelle-Institut e.V.
Johnston Jill A.
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