Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-08-09
1997-01-21
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 6, 435 724, 435 29, G01N 102, C12Q 168, C12Q 102
Patent
active
055958818
ABSTRACT:
This invention provides a novel and rapid method for isolating MHC:antigen-restricted T cells. The method comprises the steps of (a) providing an isolated MHC:peptide complex wherein said complex is bound to a solid support; (b) contacting the complex with the biological sample containing T lymphocytes to form a MHC:peptide:T cell complex; (c) removing the MHC:peptide:T cell complex from the peripheral blood; and (d) separating the bound T lymphocytes from the MHC:peptide:T cell complex. The T-cells can be used to detect the presence, in a biological sample, of antigen presenting cells bearing a preselected MHC:antigen complex.
REFERENCES:
B. Nag et al, Jour. Immunological Methods, 169, 273-285, 1994.
Van Schooten, Wim C. A., et al. (1992) "Purified HLA Class II Peptide Complexes Can Induce Adherence and Activation of Peptide-Sepcific Human T Cell Clones", The Journal of Immunology, 148:1043-1048.
Binz, Hans, et al. (1979) "Binding of Purified, Soluble Major Histocompatibility Complex Polypeptide Chains Onto Isolated T-Cell Receptors", J. Exp. Med., 150:1084-1097.
Coeshott, Claire M., et al. (1986) "Ia-Specific Mixed Leukocyte Reactive T-Cell Hybridomas: Analysis Of Their Specificity By Using Purified Class II MHC Molecules In A Synthetic Membrane System", The Journal of Immunology, 136(8):2832-2838.
Abromson-Leeman, Sara, et al. (1988) "Isolation of antigen-specific T cell clones from nonresponder mice", Eur. J. Immunol., 18:145-152.
Laundy, G. J., et al. (1989) "Applications of Automated Simultaneous Double Fluorescence (SDF). II HLA Class I Phenotyping Using Immunomagnetically Separated T Lymphocytes", Journal of Immunogenetics, 16P:141-148.
Mantovani, V., et al. (989) "Nuova Tecnica Di Purificazione Linfocitaria Per La Tipizzazione HLA", Boll. Soc. It. Biol Sper., LXV:967-973.
Kendrick Teresa
Mukku Prabha V.
Nag Bishwajit
Sharma Somesh D.
Anergen Inc.
Saunders David
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