Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Glycoproteins
Patent
1996-03-01
1998-05-12
Alexander, Lyle A.
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
Glycoproteins
436169, 422 56, G01N 3396
Patent
active
057504059
ABSTRACT:
The determination of human serum albumin (HSA) in urine is carried out by contacting the urine with a reagent system containing a buffer and a protein error indicator. Other proteins normally found in urine can compete with the HSA for interaction with the protein error indicator thereby affecting the sensitivity of the test for HSA. The present invention involves the addition of certain polymeric competitive inhibitors to the reagent system which inhibit the interaction of the urinary proteins other than HSA with the protein error indicator to a greater extent than the interaction with HSA is inhibited thereby increasing the reagent's sensitivity for HSA. Suitable polymeric inhibitors include poly(vinyl alkyl esters), poly(alkyl acrylates), poly(vinyl alkyl carbonates) and poly(vinyl alkyl ketones).
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patent: 5424215 (1995-06-01), Albarella et al.
Y. Fujita, Bunseki Kagaku (32) 379-386 (1983) "Color Reaction Between Pyrogallol Red-Molybdenum(VI) Complex and Protein".
Kragh-Hansen, Biophysics Acta, (365), 360-371 (1974) "Protein Binding of Small Molecules".
Macart et al, Clinica Chimica Acta (141), 77-84 (1984) "Evaluation of an Improved Coomassie Dye Binding Method for Urinary Protein Assay".
Albarella James P.
Cahill Sally E.
Johnson Gary M.
Pugia Michael J.
Alexander Lyle A.
Bayer Corporation
Jeffers Jerome L.
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