Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-04-30
1995-10-31
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 2432, 935 8, 935 78, C12Q 168, C07H 1700
Patent
active
054628555
ABSTRACT:
For the detection and quantitative determination of nitrosomonas strains in wastewaters and soils, a gene probe is used which, by virtue of its complementary sequences, only hybridizes with parts of the genome of nitrosomonas strains from wastewater or soil samples and does not produce a positive hybridization signal with parts of the genome of other bacteria and the hybridized nucleic acid is quantitatively determined by means of a known label of the gene probe and thus provides a direct measure of the content of nitrosomonas strains in the wastewater or soil sample.
REFERENCES:
Sommer et al., "Minimal Requirements for PCR Primers", Nucleic Acids Research, vol. 17, No. 16, 1989, p. 6749.
A. Kornberg DNA Synthesis, pp. 16-17, W. H. Freeman and Co., San Francisco (1974).
S. W. Watson and M. Mandel J. Bacteriol. 107, 563-569 (1971).
J. Meinkoth and G. M. Wahl Methods in Enzymology 152, 91-94 (1987).
STN, Registry file, Gen Bank M15861 (9CI), sequence comparison of chicken to oligonucleotide of cl. 18.
ATCC Catalogue of Bacteria and Bacteriophages, 17th Edit., 1989, p. 152.
Nucleic Acid Hybridization: A practical approach, Hames et al., Editors, IRL Press, Oxford, pp. 73, 78, 79, 94, 96, 100, 102 and 123.
Kanne Reinhard
Lobberding Antonius
Rast Hans G.
Springer Wolfgang
Bayer Aktiengesellschaft
Parr Margaret
Sisson Bradley L.
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