Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1982-07-26
1984-09-11
Padgett, Benjamin R.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
436519, 436529, 436548, 436808, 436809, 435240, 4351722, 435 68, 435 4, 435 7, 935110, G01N 3354, G01N 3358
Patent
active
044710585
ABSTRACT:
A method for the detection and/or determination of an antigen having at least two separate antibody combining sites or antigenic determinants (i.e. a polyvalent antigen) using at least two different monoclonal antibodies which bind to the separate sites is described. In particular the method utilizes Protein A with a carrier to immobilize a first monoclonal antibody which in turn, can bind to one antigenic determinant on the antigen. The second antibody with a label is provided in a solution and binds to the second antigenic determinant on the antigen. A test kit is described for practicing the method. Novel anti-PGH synthase and anti-PGI.sub.2 synthase antibodies and hybridoma cells producing such antibodies are described.
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DeWitt David L.
Smith William L.
Board of Trustees operating Michigan State University
McLeod Ian C.
Moskowitz M.
Padgett Benjamin R.
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