Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Biological or biochemical
Reexamination Certificate
2006-08-17
2010-12-14
Zeman, Mary K (Department: 1631)
Data processing: measuring, calibrating, or testing
Measurement system in a specific environment
Biological or biochemical
C702S020000, C703S011000, C435S006120, C536S024500
Reexamination Certificate
active
07853408
ABSTRACT:
A method of designing oligonucleotides useful in molecular biology techniques as PCR primers or in other techniques as identification and/or quantification probes is disclosed. The method permits designing specific oligonucleotides for the identification of a determined sequence in a metagenomic sample. The method includes selecting or constructing a database of reference sequences, selecting a subset of sequences belonging to target organisms, selecting candidate oligonucleotides from such sequences, depurating these candidate oligonucleotides according to hybridization specificity and thermodynamic stability criteria, obtaining a list of designed oligonucleotides that fulfill the hybridization specificity and thermodynamic stability criteria, producing materially by chemical synthesis the designed oligonucleotides, and selecting the oligonucleotides which comply with the requirements of the desired process.
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Aravena Duarte Andrés Octavio
Ehrenfeld Stolzenbach Katia Nicole
Gonzalez Canales Mauricio Alejandro
Maass Sepúlveda Alejandro Eduardo
Martinez Aguilera Servet
Biosigma S.A.
Merchant & Gould P.C.
Zeman Mary K
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