Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1982-12-02
1986-10-07
Rosen, Sam
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435241, C12N 500
Patent
active
046159770
ABSTRACT:
A method and medium for the multiplication of normal diploid cells from mammals is described, comprising cultivating the cells in a culture medium containing an amine derivative or derivatives represented by the general formula: NR.sup.1 R.sup.2 R.sup.3 (wherein R.sup.1, R.sup.2 and R.sup.3 represents a straight, branched or cyclic alkyl group or a hydrogen atom, provided that R.sup.1, R.sup.2 and R.sup.3 are not hydrogen atoms at the same time. Addition of these specific amine derivatives permits multiplication of normal diploid cells in a culture medium containing a limited concentration of serum. These amine derivatives are inexpensive and can be stored for a long period of time, and their storage and handling are easy. The use of these amine derivatives overcomes various problems encountered in using serum in the multiplication of normal diploid cells according to conventional methods.
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Hasegawa Akio
Horiguchi Sadakazu
Asahi Kasei Kogyo Kabushiki Kaisha
Herald William J.
Rosen Sam
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