Method for the conversion of a cellulosic substrate to glucose u

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435253, 435105, 435165, 435170, 435209, 435277, 435278, C12P 1914, C12P 1902, C12P 104, C12N 942, C12N 120, D21C 100, D21C 300

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046280294

ABSTRACT:
A method is described for the enzymatic saccharification of a cellulosic substrate to glucose which utilizes fermentation of the substrate with the cellulase enzyme complex-producing, thermophilic microorganism Microbispora bispora Rutgers P&W and its mutants. The resistance of M. bispora Rutgers P&W cellulase to end-product inhibition enables conversion efficiency superior to that of known cellulase producing microorganisms. Continued enzyme activity at elevated temperatures allows broader applicability of enzyme-catalyzed saccharification than heretofore attained. The microorganism in its purified form incubated on cellulase, and its cellulolytic mutants, are also disclosed.

REFERENCES:
patent: 3764475 (1973-10-01), Mandels
patent: 4472504 (1981-09-01), Gallo

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