Method for the controlled implementation of complex PCR...

Details Method for the controlled implementation of complex PCR... Method for the controlled implementation of complex PCR...

2006-03-07

2006-03-07

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S024300, C536S025320

Reexamination Certificate

active

07008770

ABSTRACT:
A method is described for controllably conducting complex PCR amplifications, wherein at least the following steps are conducted:a) PCR amplification with at least 50 primers of a first type (type 1) of different sequence, which are complementary to one of the strands of a random DNA sample, and also with a primer or a library of primers of a second type (type 2), which is complementary to the other strand of the DNA sample used, wherein the type 2 primers contain a first label (label 1);b) hybridizing of the amplified products to an oligomer array, which comprises oligonucleotides that hybridize to the primers utilized in the PCR reaction or to oligonucleotides that are complementary to these;or hybridizing of the amplified products to an oligomer array, which contains oligomers complementary to the primers utilized in the PCR reaction;c) length determination of the amplified products bound to the array by a second label (label 2) which can be correlated with the length of the respective DNA fragment, and which is different from the first label (label 1) in step a) andd) quantification of the signals originating from label 1 and label 2 at each site of the oligonucleotide array relevant for the analysis.

REFERENCES:
patent: 5840549 (1998-11-01), First et al.
patent: 6083701 (2000-07-01), Reeve
patent: 6653070 (2003-11-01), Olek
patent: 19801661 (1999-07-01), None
Wang et al. Science, 1998, vol. 280, p. 1077-1082.

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