Method for the continuous mass in vitro suspension culture of ce

Details Method for the continuous mass in vitro suspension culture of ce Method for the continuous mass in vitro suspension culture of ce

1975-02-11

1977-12-20

Rosen, Sam

Textiles: fiber preparation

195115, C12K 900

Patent

active

040640060

ABSTRACT:
A process for augmenting production of a specific antibody from a patient including the steps of administering a specific antigen to the patient to cause production of the specific antibody, or choosing a subject containing an antigen, e.g., cancer, or one already immunized or making antibodies to an endogenous or exogenous antigen, performing a thoracic duct fistula on the patient, raising the central venous pressure of the patient for example, by pericordial tamponde, and collecting lymph from the fistula, centrifuging the collected lymph to separate the lymph cells therein from the lymph fluid, and to form a thin elongated layer of the separated cells, treating the thin elongated layer of lymph cells to assure that they are substantially free of the specific antibody produced, dispersing the cells in a physiologically balanced saline solution and returning the dispersed cells and solution to the patient intravascularly, and giving appropriate replacement therapy to maintain normal control and health of all other systems in the body, the replacement therapy comprising fluids which can be of several kinds, but having in common that they are substantially free of the specific antibody.

REFERENCES:
patent: 3528889 (1970-09-01), Portno
patent: 3591455 (1971-07-01), Oppermann
Willmer--Cells and Tissues in Culture, vol. 2, (1965), pp. 374-376.

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