Method for the colorimetric determination of an analyte with a P

Details Method for the colorimetric determination of an analyte with a P Method for the colorimetric determination of an analyte with a P

1994-04-08

1996-01-16

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 4, 435 26, 435 25, 435118, 435119, 435120, 435 28, 435133, 436 63, C12Q 154, C12Q 100, C12P 1718, G01N 3348

Patent

active

054847087

ABSTRACT:
The invention concerns a method for the colorimetric determination of an analyte by means of enzymatic oxidation with a PQQ-dependent dehydrogenase in the presence of an electron acceptor from the group of the electron-rich aromatic nitroso compounds by enzymatic reduction of the nitroso compound to an imino compound and detection of the imino compound by color formation. In addition the invention concerns a corresponding agent containing a PQQ-dependent dehydrogenase, an electon-rich aromatic nitroso compound as well as, if desired, a detection reagent for imino compounds. The invention in addition concerns novel nitrosoamino compounds which form colored quinone diimines on reduction as well as their use for the colorimetric enzymatic determination of an analyte.

REFERENCES:
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patent: 5234818 (1993-08-01), Zimmermann et al.
patent: 5240860 (1993-03-01), Hoenes et al.
patent: 5334508 (1994-08-01), Hoenes
Chemical Abstracts, vol. 117, No. 1, (1992), #3829x.
Adachi et al., Enzymatic Determination of Pyrroloquinoline Quinone with a Quinoprotein Glycerol Dehydrogenase, Agric. Biol. Chem., 52 (8), 2081-2, 1988.

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