Chemistry: physical processes – Physical processes – Crystallization
Patent
1980-03-17
1982-01-05
Turk, Arnold
Chemistry: physical processes
Physical processes
Crystallization
23909, 260112B, G01N 3392
Patent
active
043091881
ABSTRACT:
This invention relates to a separation method comprising the introduction of a sample of serum or plasma into a microcolumn containing a support which has a sulfated polysaccharide, preferably heparin, covalently bound to it so as to remove from said sample the .beta.-lipoproteins by binding thereof to this ligand, followed by washing the microcolumn with a first pH buffered solution and collecting the eluant representing a first fraction containing the .alpha.-lipoprotein portion of the sample. Then the microcolumn is washed wth a second pH buffered solution so as to release the ligand-bound .beta.-lipoproteins and to recover a second fraction containing the .beta.-lipoprotein portion of the sample. Afterwards, it is possible to separately determine the .alpha.- and .beta.-lipoprotein-cholesterol, as well as the respective .alpha./.beta. ratios of phospholipids, triglycerides and various apoproteines, and to calculate the respective ratios .alpha./.beta., all useful as clinical diagnostic indexes and allowing to obtain a complete lipoprotein profiling.
The invention also concerns a kit comprising in a transportable box all the implements and reagents duly prepared so as to easily and quickly carry out the above method.
REFERENCES:
patent: 4039285 (1977-08-01), Teipel
patent: 4096136 (1978-06-01), Ayers et al.
patent: 4103685 (1978-08-01), Lupien et al.
patent: 4178439 (1979-12-01), Ayers et al.
Castelli et al., Circulation 55, pp. 767-772, 1977.
A. Casu, Ital. J. of Biochem. 28(1) 26, 1979.
Abstracts of the 52nd Scientific Sessions by Alanopovic et al., Abstr. American Society for Study of Atherosclerosis 33rd, 2nd, 1979.
Havel et al., J. of Cl. Invest. 34, 1345, 1955.
Eisenberg, Atherosclerosis Review vol. 1, p. 23, 1976.
Mahley et al., J. of Lipid Research 18, 314, 1977.
Lee, Science 169, 493, 1970.
Durrington et al., Clinica Chemica Acta, 71 (1976) 95-108.
Bachorik et al., Clin. Chem. 22/11, 1828-1834, 1976.
Srinivasan et al., Archives of Biochemistry and Biophysics 170, 334-340, 1975.
Warnick et al., Journal of Lipid Research 19, 65-76, 1978.
Warnick et al., Clinica Chemistry, 25, No. 4, 596, 1979.
Rhoads et al., The New England J. of Med. 294, No. 6, 293, 1976.
Gofman et al., Circulation, 34, 679, 1966.
Henry, Clinical Chemistry 2nd Ed. 1140, 1974.
Curry et al., Clinical Chemistry 24, No. 2, 280, 1978.
Symphar S.A.
Turk Arnold
LandOfFree
Method for the clinical separation of .alpha.- and .beta.-lipopr does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the clinical separation of .alpha.- and .beta.-lipopr, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the clinical separation of .alpha.- and .beta.-lipopr will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1842466