Method for the chromatographic fractionation of plasma or...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Albumin

Reexamination Certificate

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C424S176100, C424S177100, C530S390100, C530S390500, C530S415000

Reexamination Certificate

active

07041798

ABSTRACT:
The invention relates to the fractionation of plasma or serum into at least one albumin fraction and one immunoglobulin fraction by hydrophobic interaction chromatography. The fractionation is carried out using an incremental salt gradient, especially an ammonium sulfate buffer. The invention also relates to preparations obtained by using said method and to their use.

REFERENCES:
patent: 5429746 (1995-07-01), Shadle et al.
patent: 0 339 919 (1989-11-01), None
patent: 0 717 049 (1996-06-01), None
Goheen et al, Jour. of Chromatography, 326, 235-241, 1985.
Goudswaard et al, Immunochemistry, 14, 717-719, 1977.
Hirkal Z. et al; “Hydrophobic interaction chromatography of serum proteins or phenyl sepharose CL-4B”; Journal of Chromatography, vol. 242, No. 2 (1982); pp. 385-388.

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