Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-02-13
2007-02-13
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C435S091520
Reexamination Certificate
active
09673739
ABSTRACT:
The present invention is drawn to a method for characterising nucleic acid molecules, which comprises the steps of: i) introducing a modified base which is a substrate for a DNA glycosylase into a DNA molecule; ii) excising the modified base with the DNA glycosylase to generate an abasic site; iii) cleaving the DNA at the abasic site to generate and release an extendible upstream DNA fragment having a 3′ hydroxyl terminus; and iv) incubating the released extendible upstream DNA fragment in the presence of an enzyme allowing for extension thereof and an additional template nucleic acid and analysing resultant fragment(s).
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McGrath, A. et al. “A DNA glycolase-based fingerprint for accurate identification of amplified DNA products and its application in the Accurate diagnosis of infectious organisms”, Analytical Biochemistry, vol. 259, No. 2, Jun. 1998, pp. 288-292.
McCarthy Thomas Valentine
Vaughan Patrick Martin
Birch & Stewart Kolasch & Birch, LLP
Enterprise Ireland (T/A BioResearch Ireland)
Horlick Kenneth R.
Tung Joyce
University College Cork Natl. University of Ireland
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